molecular weight markers  (Vector Laboratories)

Journal: Biomedicines

Article Title: External Support of Autologous Internal Jugular Vein Grafts with FRAME Mesh in a Porcine Carotid Artery Model

doi: 10.3390/biomedicines12061335

Figure Lengend Snippet: Microscopical examinations of autologous internal jugular vein interposition grafts in porcine carotid arteries at one month post implantation (control group). Representative cross-sections of the midgraft regions are shown in Pigs 2 ( A – D and U – X ), 3 ( E – H ), 4 ( M – P ), 5 ( Y – Z2 ), 6 ( I – L ), and 7 ( Q – T ). The seven control samples are ordered from left to right according to degree of pathological remodeling and vein graft lesions, i.e., patent, mildly stenotic, severely stenotic, or totally occluded (see for more details). We performed histological staining with hematoxylin and eosin and Weigert van Gieson and Resorcin-fuchsin (elastica), as well as immunohistochemistry of alpha-smooth muscle (SM) actin and endothelial markers ERG ( D , L , P , X ) or CD31 ( H , T , Z2 ). Magnification was 20×.

Article Snippet: We carried out immunostaining for the expression of alpha-smooth muscle actin (primary antibody: REF: 202M, Cell Marque Corp., a part of MilliporeSigma, Rocklin, CA, USA; secondary antibody: provided as part of the UltraView Universal DAB Detection Kit, REF: 760–500, Ventana Medical Systems, Inc., Roche Group, Tucson, AZ, USA), endothelial marker CD31 (primary antibody: REF: NB100-2284, Novus Biologicals, LLC, Centennial, CO, USA; secondary antibody: REF: BA-1000, Vector Laboratories, Inc., Burlingame, CA, USA), and endothelial marker ERG (primary antibody: REF: 434R, Cell Marque Corp., a part of MilliporeSigma, Rocklin, CA, USA; secondary antibody: provided as part of the OptiView DAB IHC Detection Kit, REF: 760–700, Ventana Medical Systems, Inc., Roche Group, Tucson, AZ, USA).

Techniques: Control, Staining, Immunohistochemistry